Virginie Mournetas, PhD
Systems Biology
Welcome!
This is my personal website.
I am a systems biology/pluripotent stem cell biologist.
I have discovered the research world during my first internship in 2009 in Japan.
Since then, I have developed my skills in the field of stem cells and bioinformatics.
I particularly enjoy to work on multidisciplinary approaches mixing biology, informatics and mathematics.
I did my 4-year PhD at the University of Liverpool (UK), a first 4.5-year postdoc at I-Stem (Genopole, Evry, France) and a second one at TIMC-EHPP (UGA, Grenoble, France).
I am now working with ADLIN Science.
Feel free to contact me!
My research interests
The main ones
#StemCells
#SystemsBiology
#omics
#OpenAccess
#DataVisualisation
The current ones
#Dockers
#DataIntegration
#IA
The former ones
#skin
#exposome
#aging
#KnockDown
#lentivirus
#RNAinterference
#dystrophin
#DMD
#ExonSkipping
#HighThroughputScreening
Latest news
#Muscle #DMD #myogenesis #multiOmics #WebApp
I am very pleased to share with you our latest work on
human myogenesis
and
DMD early onset
modelling.
We have analysed
time series omics
on
human pluripotent derived cells
and found several interesting elements around mitochondria or fibrosis.
But to us, the most important finding is that transcriptional defects appear at the somite stage, before the expression of skeletal muscle markers and before the expression of the muscular dystrophin.
If you are interested, you can find the entire manuscript
online
, as well as explore the entire datasets via a
Shiny app.
Feel free to contact us for any questions or comments you have; we will be happy to discuss.
Upcoming presentations
Research projects
Keywords: Skin, Ageing, Exposome, Multi-omics, Epigenetics
Publications:
Publications:
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, human pluripotent stem cells, omics
Publications:
Publications:
- Duchenne muscular dystrophy: a developmental disease? / La dystrophie musculaire de Duchenne : une maladie du développement ?
- Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset
- Slides: A multi-omics graphical interface for exploring DMD onset and human muscle development
- Slides: Seeing Duchenne muscular dystrophy as developmental disease
- Slides: Pluripotent stem cells applied to DMD: A skeletal muscle model, a developmenral model, a screening model
- Poster: Duchenne muscular dystrophy in a dish using human pluripotent stem cells: a model effciently recapitulating the disease phenotype
- Poster: Duchenne muscular dystrophy, a progressive developmental disease
- Bulk mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8321
- Bulk miR-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8293
- Single-cell mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-9510
- TMT Isobaric quantitative proteomics: deposited in the PRIDE Archive database at EMBL-EBI under accession number PXD015355
Background
Duchenne muscular dystrophy (DMD) causes severe disability of children and death of young men, with an incidence of approximately 1/5000 male births. Symptoms appear in early childhood, with a diagnosis made mostly around 4 years old, a time where the amount of muscle damage is already significant, preventing early therapeutic interventions that could be more efficient at halting disease progression. In the meantime, the precise moment at which disease phenotypes arise—even asymptomatically—is still unknown. Thus, there is a critical need to better define DMD onset as well as its first manifestations, which could help identify early disease biomarkers and novel therapeutic targets.
Methods
We have used both human tissue-derived myoblasts and human induced pluripotent stem cells (hiPSCs) from DMD patients to model skeletal myogenesis and compared their differentiation dynamics with that of healthy control cells by a comprehensive multi-omic analysis at seven time points. Results were strengthened with the analysis of isogenic CRISPR-edited human embryonic stem cells and through comparisons against published transcriptomic and proteomic datasets from human DMD muscles. The study was completed with DMD knockdown/rescue experiments in hiPSC-derived skeletal muscle progenitor cells and adenosine triphosphate measurement in hiPSC-derived myotubes.
Results
Transcriptome and miRnome comparisons combined with protein analyses demonstrated that hiPSC differentiation (i) leads to embryonic/foetal myotubes that mimic described DMD phenotypes at the differentiation endpoint and (ii) homogeneously and robustly recapitulates key developmental steps—mesoderm, somite, and skeletal muscle. Starting at the somite stage, DMD dysregulations concerned almost 10% of the transcriptome. These include mitochondrial genes whose dysregulations escalate during differentiation. We also describe fibrosis as an intrinsic feature of DMD skeletal muscle cells that begins early during myogenesis. All the omics data are available online for exploration through a graphical interface at https://muscle-dmd.omics.ovh/.
Conclusions
Our data argue for an early developmental manifestation of DMD whose onset is triggered before the entry into the skeletal muscle compartment, data leading to a necessary reconsideration of dystrophin roles during muscle development. This hiPSC model of skeletal muscle differentiation offers the possibility to explore these functions as well as find earlier DMD biomarkers and therapeutic targets.
Duchenne muscular dystrophy (DMD) causes severe disability of children and death of young men, with an incidence of approximately 1/5000 male births. Symptoms appear in early childhood, with a diagnosis made mostly around 4 years old, a time where the amount of muscle damage is already significant, preventing early therapeutic interventions that could be more efficient at halting disease progression. In the meantime, the precise moment at which disease phenotypes arise—even asymptomatically—is still unknown. Thus, there is a critical need to better define DMD onset as well as its first manifestations, which could help identify early disease biomarkers and novel therapeutic targets.
Methods
We have used both human tissue-derived myoblasts and human induced pluripotent stem cells (hiPSCs) from DMD patients to model skeletal myogenesis and compared their differentiation dynamics with that of healthy control cells by a comprehensive multi-omic analysis at seven time points. Results were strengthened with the analysis of isogenic CRISPR-edited human embryonic stem cells and through comparisons against published transcriptomic and proteomic datasets from human DMD muscles. The study was completed with DMD knockdown/rescue experiments in hiPSC-derived skeletal muscle progenitor cells and adenosine triphosphate measurement in hiPSC-derived myotubes.
Results
Transcriptome and miRnome comparisons combined with protein analyses demonstrated that hiPSC differentiation (i) leads to embryonic/foetal myotubes that mimic described DMD phenotypes at the differentiation endpoint and (ii) homogeneously and robustly recapitulates key developmental steps—mesoderm, somite, and skeletal muscle. Starting at the somite stage, DMD dysregulations concerned almost 10% of the transcriptome. These include mitochondrial genes whose dysregulations escalate during differentiation. We also describe fibrosis as an intrinsic feature of DMD skeletal muscle cells that begins early during myogenesis. All the omics data are available online for exploration through a graphical interface at https://muscle-dmd.omics.ovh/.
Conclusions
Our data argue for an early developmental manifestation of DMD whose onset is triggered before the entry into the skeletal muscle compartment, data leading to a necessary reconsideration of dystrophin roles during muscle development. This hiPSC model of skeletal muscle differentiation offers the possibility to explore these functions as well as find earlier DMD biomarkers and therapeutic targets.
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, exon skipping, human pluripotent stem cells, high throughput screening
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
- Slides (English): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Slides (French): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Video: (French) Saga Jeunes talents - Virginie du Génopole
- Video: (French) MT180 presentation on Duchenne myopathy and exon skipping strategy
Keywords: hESC, pluripotency, differentiation, transcriptome, interactome, RT-qPCR, shRNA, lentivirus, immunofluorescence, Western blot
Publications:
Publications:
- Thesis: Defining stemness of human embryonic stem cells: a systems biology approach
- Network based meta-analysis prediction of microenvironmental relays involved in stemness of human embryonic stem cells
- A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
- Slides: In silico approach to define stemness in human embryonic stem cells
- Slides: Mercia Stem Cell Alliance workshop on lentivirus
- Slides: Back to the stemhood (teaser talk)
- Poster: In silico approach to define stemness in human embryonic stem cells
- Poster: Back to the stemhood
Human embryonic stem cells (hESCs) come from the inner cell mass of in vitro fertilized blastocysts. These pluripotent cells are transiently present in vivo and give rise to the three germ layers (endoderm, mesoderm, ectoderm). in vitro, they can be maintained undifferentiated or committed into lineages under specific culture conditions. At present, little is known about the mechanisms that regulate hESC stemness; in particular, the role of cell-cell and cell-matrix interactions has been relatively unexplored.
We propose a top-down approach to identify new extracellular proteins responsible for hESC stemness maintenance. The first step consists of an in silico study, combining the analysis of microarrays and the use of annotated databases, to build and define a hESC transcriptome (8,934 messenger RNAs) and interactome (more than 6,000 proteins and almost 47,000 interactions). This interactome or protein-protein interaction network has been structurally studied and filtered to narrow down it to a short list of 92 candidate proteins, focusing on the extracellular matrix molecules and their links to the transcription factor network. Some of these 92 proteins are already known to be involved in hESC 'stemness' whereas most of them are not yet. The second step comprises an in vitro study where few of these candidates have been selected and their role in maintaining hESC stemness is being assessed. To this end, shRNA technology is used to knockdown candidate gene expression, and the effect this has on the expression of pluripotency markers is investigated using at the mRNA by qPCR and at the protein level by immunofluorescence and Western Blotting.
This Systems Biology approach should bring new clues for a better understanding of the stemness state in order to mobilise the great potential of hESCs.
We propose a top-down approach to identify new extracellular proteins responsible for hESC stemness maintenance. The first step consists of an in silico study, combining the analysis of microarrays and the use of annotated databases, to build and define a hESC transcriptome (8,934 messenger RNAs) and interactome (more than 6,000 proteins and almost 47,000 interactions). This interactome or protein-protein interaction network has been structurally studied and filtered to narrow down it to a short list of 92 candidate proteins, focusing on the extracellular matrix molecules and their links to the transcription factor network. Some of these 92 proteins are already known to be involved in hESC 'stemness' whereas most of them are not yet. The second step comprises an in vitro study where few of these candidates have been selected and their role in maintaining hESC stemness is being assessed. To this end, shRNA technology is used to knockdown candidate gene expression, and the effect this has on the expression of pluripotency markers is investigated using at the mRNA by qPCR and at the protein level by immunofluorescence and Western Blotting.
This Systems Biology approach should bring new clues for a better understanding of the stemness state in order to mobilise the great potential of hESCs.
Keywords: LIF, mESC, pluripotency, differentiation, transcriptome, proteome, Lifind genes, RT-qPCR, siRNA, iTRAQTM, MS
Understanding the action mechanisms of LIF (Leukemia Inhibitory Factor), a cytokine with pleiotropic effects involved in maintenance of pluripotency in mESC, is the objective of my reception laboratory (UMR CNRS 5164 CIRID). The model is composed of 3 states: i) the pluripotency state with LIF-dependent gene expression, ii) the reversible and iii) irreversible commitment toward differentiation where the role of LIF-induced genes have to be defined. A transcriptomic analysis allowed the identification of a cluster of genes named Lifind (for LIF induced) whose expression is induced by addition of LIF after 24 or 48 hours of culture without this cytokine. These conditions correspond to two cell states in which LIF has different effetcs.
The purpose of my placement was to complete the transcriptomic analysis by i) developing a functional test based on siRNA/RT-qPCR strategy to study effects of Lifind genes on pluripotent and committed cells and ii) a proteomic approach (in collaboration with the proteomic platform of Bordeaux2 University), by iTRAQTM labelling and mass spectrometry, to identify and quantify most proteins of total cellular lysates obtained from pluripotent or committed cells.
Functional test and proteomic analysis revealed to be first conclusive approaches respectively for the study of Lifind genes and for the comparison of proteomes.
The purpose of my placement was to complete the transcriptomic analysis by i) developing a functional test based on siRNA/RT-qPCR strategy to study effects of Lifind genes on pluripotent and committed cells and ii) a proteomic approach (in collaboration with the proteomic platform of Bordeaux2 University), by iTRAQTM labelling and mass spectrometry, to identify and quantify most proteins of total cellular lysates obtained from pluripotent or committed cells.
Functional test and proteomic analysis revealed to be first conclusive approaches respectively for the study of Lifind genes and for the comparison of proteomes.
Keywords: lipoxygenase, moss, cloning, sequencing
The Department of Life Science and Biotechnology, which is part of the Faculty of Life and Environmental Science of Shimane University at Matsue (Japan), studies lipids. Cloning, sequencing and characterisation of novel moss lipoxygenases is one of its research focus.
Lipoxygenases were found in Phiscomitrella patens moss and my reception laboratory was searching for novel lipoxygenases in Ricciocarpos natans moss. During my placement, I cloned and sequenced pieces of lypoxygenases. ChromasPro freeware allowed the sequencing analysis. Some portions of a novel lipoxygenase were determined, in particular some restriction sites. Presence of other lipoxygenases into realised clones are to be confirmed.
Lipoxygenases were found in Phiscomitrella patens moss and my reception laboratory was searching for novel lipoxygenases in Ricciocarpos natans moss. During my placement, I cloned and sequenced pieces of lypoxygenases. ChromasPro freeware allowed the sequencing analysis. Some portions of a novel lipoxygenase were determined, in particular some restriction sites. Presence of other lipoxygenases into realised clones are to be confirmed.
Full list of oral presentations
- 03/2012 - UK - Liverpool Postgraduate Symposium - In silico approach to define ‘stemness’ in human embryonic stem cells - 10 minutes
- 05/2012 - UK - Manchester Life Sciences PhD Conference - In silico approach to define ‘stemness’ in human embryonic stem cells - 20 minutes
- 06/2012 - FRANCE - Invited by Hélène Boeuf, CNRS UMR CNRS 5164 CIRID, Bordeaux - A Systems Biology approach to define ‘stemness’ in human embryonic stem cells - 40 minutes
- 01/2013 - UK - Institute of Integrative Biology, Research and Impact Day, Liverpool - Back to the stemhood - a Systems Biology approach identifying external relays involved in stemness of human embryonic stem cells - 2-minute teaser talk
- 09/2013 - UK - PPI-Net Young Researchers Meeting, Leed - Defining stemness of human embryonic stem cells: A systems biology approach - 15 minutes
- 02/2014 - UK - Departmental presentation, Liverpool - Defining stemness of human embryonic stem cells: A systems biology approach - 40 minutes
- 09/2014 - UK - Workshop presentation, Liverpool - Lentivirus approach for knock-in/knock-down studies - 1 hour presentation, co-presented with Sofia Melo Pereira
- 07/2015 - UK - Invited by TrishMurray, the Physiology department, University of Liverpool - Duchenne muscular dystrophy - Human pluripotent stem cells & Exon skipping strategy - 40 minutes
- 11/2015 - FRANCE - Opération "1000 Chercheurs dans les Ecoles", Scientific popularisation in high schools- 1 hour presentation * 8 times - Press articles (in French): L-Echo.info & La Montagne
- 02/2016 - FRANCE - The 5th annual consortium meeting of Revive (Regenerative biology & medicine), Chantilly-Gouvieux - Human pluripotent stem cells: Tools to understand Duchenne myopathies and propose novel therapeutic approaches - 1-minute teaser talk
- 06/2016 - FRANCE - Genoforum, Evry - Duchenne muscular dystrophy and exon skipping strategy - 3-minute presentation in a 3MT style (Video & Slides)
- 09/2016 - FRANCE - Forum BiotechPolytech'Marseille - Human pluripotent stem cells: Tools to understand Duchenne muscular dystrophy (DMD) & develop therapeutic approaches - 40 minutes
- 10/2016 - FRANCE - 2nd workshop onRegenerative Medicine, Bordeaux - Human pluripotent stem cells: Tools to understand Duchenne muscular dystrophy (DMD) & develop therapeutic approaches - 10 minutes (best oral presentation prize)
- 11/2016 - FRANCE - I-Stem, Corbeil-Essonnes - Project presentation to the Crédit Agricole - Scientific popularisation - 30 minutes
- 11/2016 - FRANCE - Opération "1000 Chercheurs dans les Ecoles", Scientific popularisation in high schools
- 01/2017 - FRANCE - The 6th annual consortium meeting of Revive (Regenerative biology & medicine), Chantilly-Gouvieux - Defining an early phenotype of Duchenne muscular dystrophy (DMD): a systems biology approach using human pluripotent stem cells - 15 minutes
- 02/2017 - FRANCE - External seminar Ecole Polytech’Clermont-Ferrand - 40 minutes
- 04/2017 - FRANCE - BioTherAlliance meeting – 10 minutes
- 11/2017 - FRANCE - Opération "1000 Chercheurs dans les Ecoles", Scientific popularisation in high schools
- 01/2018 - FRANCE - The 7th annual consortium meeting of Revive (Regenerative biology & medicine), Chantilly-Gouvieux - Duchenne muscular dystrophy: a developmental disease? - 15 minutes
- 11/2018 - FRANCE - Invited by Chantal Housset, Faculté de médecine Pierre et Marie Curie, Paris - Duchenne muscular dystrophy: a human developmental disease - 45 minutes
- 11/2018 - FRANCE - Opération "1000 Chercheurs dans les Ecoles", Scientific popularisation in high schools
- 03/2019 - FRANCE - Invited David Israeli, Genethon, Evry - Duchenne muscular dystrophy: a developmental disease - 45 minutes
- 03/2019 - FRANCE - Invited by Hélène Boeuf, BxCRM, Bordeaux - Duchenne muscular dystrophy: a developmental disease - 45 minutes
- 04/2019 - FRANCE - The 8th annual consortium meeting of Revive (Regenerative biology & medicine), Chantilly-Gouvieux - Duchenne muscular dystrophy: a developmental disease - 15 minutes
- 04/2019 - UK - Invited by Trish Murray, the Physiology department, University of Liverpool - Duchenne muscular dystrophy: a developmental disease - 40 minutes
- 06/2019 - USA - 1st France-USA Stem cell Symposium, Los Angeles - Duchenne muscular dystrophy: a developmental disease
- - 10 minutes
- 07/2019 - FRANCE - Invited by Laurent Schaeffer, the NeuroMyoGene Institute, Lyon - Duchenne muscular dystrophy: a developmental disease - 40 minutes
- 07/2019 - FRANCE - Aurastem meeting, Lyon - Duchenne muscular dystrophy: a developmental disease - 10 minutes
- 09/2019 - JAPAN - Invited by Shin'ichi Takeda, National Institute of neuroscience, Tokyo - Duchenne muscular dystrophy: a developmental disease - Myogenesis modelled by human pluripotent stem cells uncovers Duchenne muscular dystrophy phenotypes prior to skeletal muscle commitment - 40 minutes
- 09/2019 - FRANCE - Invited by Pascal Maire/Athanassia Sotiropoulos, Institut Cochin, Paris - Duchenne muscular dystrophy: a developmental disease - Myogenesis modelled by human pluripotent stem cells uncovers Duchenne muscular dystrophy phenotypes prior to skeletal muscle commitment - 40 minutes
- 10/2019 - FRANCE - Invited by Frederic Relaix, Université Pari-est, Créteil - Duchenne muscular dystrophy: a developmental disease - Myogenesis modelled by human pluripotent stem cells uncovers Duchenne muscular dystrophy phenotypes prior to skeletal muscle commitment - 40 minutes
- 11/2019 - FRANCE - Workshop “Computational systems biology for complex diseases” 2019, Paris-Saclay - Duchenne muscular dystrophy: a developmental disease - Myogenesis modelled by human pluripotent stem cells uncovers Duchenne muscular dystrophy phenotypes prior to skeletal muscle commitment - 1 hour
- 02/2020 - FRANCE - Invited by Nicolas Glade, TIMC-IMAG, Grenoble - Duchenne muscular dystrophy: a developmental disease - Myogenesis modelled by human pluripotent stem cells uncovers Duchenne muscular dystrophy phenotypes prior to skeletal muscle commitment - 1 hour
Examples of oral presentations
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, human pluripotent stem cells, omics
Publications:
Publications:
- Duchenne muscular dystrophy: a developmental disease? / La dystrophie musculaire de Duchenne : une maladie du développement ?
- Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset
- Slides: A multi-omics graphical interface for exploring DMD onset and human muscle development
- Slides: Seeing Duchenne muscular dystrophy as developmental disease
- Slides: Pluripotent stem cells applied to DMD: A skeletal muscle model, a developmenral model, a screening model
- Poster: Duchenne muscular dystrophy in a dish using human pluripotent stem cells: a model effciently recapitulating the disease phenotype
- Poster: Duchenne muscular dystrophy, a progressive developmental disease
- Bulk mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8321
- Bulk miR-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8293
- Single-cell mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-9510
- TMT Isobaric quantitative proteomics: deposited in the PRIDE Archive database at EMBL-EBI under accession number PXD015355
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, exon skipping, human pluripotent stem cells, high throughput screening
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
- Slides (English): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Slides (French): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Video: (French) Saga Jeunes talents - Virginie du Génopole
- Video: (French) MT180 presentation on Duchenne myopathy and exon skipping strategy
Project 02
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, human pluripotent stem cells, omics
Publications:
Publications:
- Duchenne muscular dystrophy: a developmental disease? / La dystrophie musculaire de Duchenne : une maladie du développement ?
- Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset
- Slides: A multi-omics graphical interface for exploring DMD onset and human muscle development
- Slides: Seeing Duchenne muscular dystrophy as developmental disease
- Slides: Pluripotent stem cells applied to DMD: A skeletal muscle model, a developmenral model, a screening model
- Poster: Duchenne muscular dystrophy in a dish using human pluripotent stem cells: a model effciently recapitulating the disease phenotype
- Poster: Duchenne muscular dystrophy, a progressive developmental disease
- Bulk mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8321
- Bulk miR-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8293
- Single-cell mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-9510
- TMT Isobaric quantitative proteomics: deposited in the PRIDE Archive database at EMBL-EBI under accession number PXD015355
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, exon skipping, human pluripotent stem cells, high throughput screening
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
- Slides (English): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Slides (French): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Video: (French) Saga Jeunes talents - Virginie du Génopole
- Video: (French) MT180 presentation on Duchenne myopathy and exon skipping strategy
Project 02
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, human pluripotent stem cells, omics
Publications:
Publications:
- Duchenne muscular dystrophy: a developmental disease? / La dystrophie musculaire de Duchenne : une maladie du développement ?
- Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset
- Slides: A multi-omics graphical interface for exploring DMD onset and human muscle development
- Slides: Seeing Duchenne muscular dystrophy as developmental disease
- Slides: Pluripotent stem cells applied to DMD: A skeletal muscle model, a developmenral model, a screening model
- Poster: Duchenne muscular dystrophy in a dish using human pluripotent stem cells: a model effciently recapitulating the disease phenotype
- Poster: Duchenne muscular dystrophy, a progressive developmental disease
- Bulk mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8321
- Bulk miR-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8293
- Single-cell mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-9510
- TMT Isobaric quantitative proteomics: deposited in the PRIDE Archive database at EMBL-EBI under accession number PXD015355
English
French
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, exon skipping, human pluripotent stem cells, high throughput screening
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
- Slides (English): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Slides (French): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Video: (French) Saga Jeunes talents - Virginie du Génopole
- Video: (French) MT180 presentation on Duchenne myopathy and exon skipping strategy
Keywords: hESC, pluripotency, differentiation, transcriptome, interactome, RT-qPCR, shRNA, lentivirus, immunofluorescence, Western blot
Publications:
Publications:
- Thesis: Defining stemness of human embryonic stem cells: a systems biology approach
- Network based meta-analysis prediction of microenvironmental relays involved in stemness of human embryonic stem cells
- A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
- Slides: In silico approach to define stemness in human embryonic stem cells
- Slides: Mercia Stem Cell Alliance workshop on lentivirus
- Slides: Back to the stemhood (teaser talk)
- Poster: In silico approach to define stemness in human embryonic stem cells
- Poster: Back to the stemhood
Keywords: hESC, pluripotency, differentiation, transcriptome, interactome, RT-qPCR, shRNA, lentivirus, immunofluorescence, Western blot
Publications:
Publications:
- Thesis: Defining stemness of human embryonic stem cells: a systems biology approach
- Network based meta-analysis prediction of microenvironmental relays involved in stemness of human embryonic stem cells
- A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
- Slides: In silico approach to define stemness in human embryonic stem cells
- Slides: Mercia Stem Cell Alliance workshop on lentivirus
- Slides: Back to the stemhood (teaser talk)
- Poster: In silico approach to define stemness in human embryonic stem cells
- Poster: Back to the stemhood
Keywords: hESC, pluripotency, differentiation, transcriptome, interactome, RT-qPCR, shRNA, lentivirus, immunofluorescence, Western blot
Publications:
Publications:
- Thesis: Defining stemness of human embryonic stem cells: a systems biology approach
- Network based meta-analysis prediction of microenvironmental relays involved in stemness of human embryonic stem cells
- A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
- Slides: In silico approach to define stemness in human embryonic stem cells
- Slides: Mercia Stem Cell Alliance workshop on lentivirus
- Slides: Back to the stemhood (teaser talk)
- Poster: In silico approach to define stemness in human embryonic stem cells
- Poster: Back to the stemhood
Full list of posters
- 12/2011 - UK - Mercia Stem Cell Alliance, Annual Scientific Meeting, Manchester - In silico approach to define ‘stemness’ in human embryonic stem cells
- 01/2012 - UK - Institute of Integrative Biology, Research and Impact Day, Liverpool - In silico approach to define ‘stemness’ in human embryonic stem cells
- 05/2012 - SWITZERLAND - Gordon Research Conference on Fibroblast GrowthFactors in Development & Disease, Les Diablerets - In silico approach to define ‘stemness’ in human embryonic stem cells (co-best poster prize)
- 12/2012 - UK - Mercia Stem Cell Alliance, Annual Scientific Meeting, Nottingham - Identification and study of proteins potentially involved in ‘stemness’ maintenance of human embryonic stem cells: a top-down approach
- 01/2013 - UK - Institute of Integrative Biology, Research and Impact Day, Liverpool - Back to the stemhood - a Systems Biology approach identifying external relays involved in stemness of human embryonic stem cells
- 05/2013 - UK - Manchester Life Sciences PhD Conference - Back to the stemhood - a Systems Biology approach identifying external factors involved in stemness of human embryonic stem cells
- 10/2013 - GERMANY - Quantitative Single Cell Biology in Stem Cell Researchmeeting, Munich - Back to the stemhood - a Systems Biology approach identifying external factors involved in stemness of human embryonic stem cells
- 12/2013 - UK - Mercia Stem Cell Alliance, Annual Scientific Meeting, University of Keele - Back to the stemhood - a Systems Biology approach to identify extracellular factors involved in stemness of human embryonic stem cells
- 01/2014 - UK - Institute of Integrative Biology, Research and Impact Day, Liverpool - Back to the stemhood - a Systems Biology approach to identify extracellular factors involved in stemness of human embryonic stem cells (best poster prize)
- 04/2014 - UK - Institute of Translational Medicine, Post-Graduate Symposium, Liverpool - Back to the stemhood - a Systems Biology approach to identify extracellular factors involved in stemness of human embryonic stem cells (best poster prize)
- 06/2015 - FRANCE - Colloques JeunesChercheurs, Journées des Familles 2015, Paris - Duchenne muscular dystrophy: pluripotent stem cells & exon skipping strategy
- 02/2016 - FRANCE - The 5th annual consortium meeting of Revive (Regenerativebiology & medicine), Chantilly-Gouvieux - Human pluripotent stem cells: Tools to understand Duchenne myopathies and propose novel therapeutic approaches
- 03/2016 - FRANCE - Myology 2016, 5th International Congress of Myology, Lyon - Duchenne muscular dystrophy: an easy, fast and robust cell platform for developing therapeutic strategies
- 10/2016 - FRANCE - iForum 2016 - Connect Discover Innovate, Paris - Human pluripotent stem cells: Tools to understand Duchenne muscular dystrophy (DMD) & develop therapeutic approaches
- 01/2017 - FRANCE - The 6th annual consortium meeting of Revive (Regenerativebiology & medicine), Chantilly-Gouvieux
- 11/2017 - FRANCE - Annual meeting of the French Society of Myology (SFM), Colmar
- 11/2017 - FRANCE - Annual meeting of the French Society for Stem CellResearch (FSSCR), Paris
- 01/2018 - FRANCE - The 7th annual consortium meeting of Revive (Regenerativebiology & medicine), Chantilly-Gouvieux
- 12/2018 - FRANCE - Annual meeting of the French Society for Stem Cell Research (FSSCR), Nantes
- 12/2018 - FRANCE - Single-cell meeting, Curie Institute, Paris
- 03/2019 - FRANCE - Myology 2019, 6th International Congress of Myology, Bordeaux
- 06/2019 - FRANCE - Maladies Rares congress, Paris
- 06/2019 - USA - ISSCR annual meeting, Los Angeles
- 11/2019 - FRANCE - Annual meeting of the French Society for Stem Cell Research (FSSCR), Lyon
- 11/2019 - FRANCE - Annual meeting of the French Society of Myology (SFM), Marseille
Examples of posters
Project 01
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, exon skipping, human pluripotent stem cells, high throughput screening
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
- Slides (English): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Slides (French): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Video: (French) Saga Jeunes talents - Virginie du Génopole
- Video: (French) MT180 presentation on Duchenne myopathy and exon skipping strategy
Project 02
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, human pluripotent stem cells, omics
Publications:
Publications:
- Duchenne muscular dystrophy: a developmental disease? / La dystrophie musculaire de Duchenne : une maladie du développement ?
- Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset
- Slides: A multi-omics graphical interface for exploring DMD onset and human muscle development
- Slides: Seeing Duchenne muscular dystrophy as developmental disease
- Slides: Pluripotent stem cells applied to DMD: A skeletal muscle model, a developmenral model, a screening model
- Poster: Duchenne muscular dystrophy in a dish using human pluripotent stem cells: a model effciently recapitulating the disease phenotype
- Poster: Duchenne muscular dystrophy, a progressive developmental disease
- Bulk mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8321
- Bulk miR-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-8293
- Single-cell mRNA-seq: deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-9510
- TMT Isobaric quantitative proteomics: deposited in the PRIDE Archive database at EMBL-EBI under accession number PXD015355
Keywords: hESC, pluripotency, differentiation, transcriptome, interactome, RT-qPCR, shRNA, lentivirus, immunofluorescence, Western blot
Publications:
Publications:
- Thesis: Defining stemness of human embryonic stem cells: a systems biology approach
- Network based meta-analysis prediction of microenvironmental relays involved in stemness of human embryonic stem cells
- A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
- Slides: In silico approach to define stemness in human embryonic stem cells
- Slides: Mercia Stem Cell Alliance workshop on lentivirus
- Slides: Back to the stemhood (teaser talk)
- Poster: In silico approach to define stemness in human embryonic stem cells
- Poster: Back to the stemhood
Keywords: hESC, pluripotency, differentiation, transcriptome, interactome, RT-qPCR, shRNA, lentivirus, immunofluorescence, Western blot
Publications:
Publications:
- Thesis: Defining stemness of human embryonic stem cells: a systems biology approach
- Network based meta-analysis prediction of microenvironmental relays involved in stemness of human embryonic stem cells
- A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
- Slides: In silico approach to define stemness in human embryonic stem cells
- Slides: Mercia Stem Cell Alliance workshop on lentivirus
- Slides: Back to the stemhood (teaser talk)
- Poster: In silico approach to define stemness in human embryonic stem cells
- Poster: Back to the stemhood
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, exon skipping, human pluripotent stem cells, high throughput screening
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
- Slides (English): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Slides (French): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Video: (French) Saga Jeunes talents - Virginie du Génopole
- Video: (French) MT180 presentation on Duchenne myopathy and exon skipping strategy
Keywords: Duchenne myopathy, Duchenne muscular dystrophy, dystrophin, exon skipping, human pluripotent stem cells, high throughput screening
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
Publications: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Presentations:
- Slides (English): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Slides (French): 3MT (English) / MT180 (French) presentation on Duchenne myopathy and exon skipping strategy
- Video: (French) Saga Jeunes talents - Virginie du Génopole
- Video: (French) MT180 presentation on Duchenne myopathy and exon skipping strategy
Under construction
2015-2019 - DMD project
Muscle-DMD-omics app
Peer-reviewed articles
9
- 2022
-
Cells . doi.org/10.3390/cells11244098
(previously submitted as preprint to bioRxiv - August 2021)
M. Mavinga, M. Palmier, M. Rémy, C. Jeannière, S. Lenoir, S. Rey, M. Saint-Marc, F. Alonso, E. Génot, N. Thébaud, E. Chevret, V. Mournetas , B. Rousseau, C. Boiziau, H. Boeuf - 2022
-
Plos One . doi.org/10.1371/journal.pone.0271847
(previously submitted as preprint to bioRxiv - July 2022)
S. Auger, V. Mournetas , H. Chiapello, V. Loux, P. Langella, J.-M. Chatel - 2022
-
eLife . doi.org/10.7554/eLife.75521
(previously submitted as preprint to bioRxiv - August 2021)
M.R.F. Gosselin, V. Mournetas , M. Borczyk, S. Verma, A. Occhipinti, J. Róg, L. Bozycki, M. Korostynski, S. C Robson, C. Angione, C. Pinset, D.C. Gorecki - 2021
-
Journal of Cachexia, Sarcopenia and Muscle . doi.org/10.1002/jcsm.12665
(previously submitted as preprint to bioRxiv - August 2019)
V. Mournetas , E. Massouridès, J.B. Dupont, H. Polvèche, M. Jarrige, A.R.L. Dorval, M.R.F. Gosselin, A. Manousopoulou, S.D. Garbis, D.C. Górecki, C. Pinset - 2019
-
Nature Communications . doi:10.1038/s41467-018-07845-z
(previously submitted as preprint to bioRxiv - October 2017)
P.E. Mangeot, V. Rissons, F. Fusil, A. Marnef, E. Laurent, J. Blin, V. Mournetas , E. Massouridès, T.J.M. Sohier, A. Corbin, F. Aube, C. Pinset, L. Schaeffer, G. Legube, F.-L. Cosset, E. Verhoey, T. Ohlmann, E. P. Ricci - 2016
-
JBM . DOI: 10.14440/jbm.2016.142
V. Mournetas , S. Melo Pereira, D. G. Fernig, P. A. Murray -
Plos One . DOI: 10.1371/journal.pone.0146281
A. A. Hammoud, N. Kirstein, V. Mournetas , A. Darracq, S. Broc, C. Blanchard, D. Zeineddine, M. Mortada, H. Bœuf - 2015
-
Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Skeletal Muscle . DOI:10.1186/s13395-015-0062-6
E. Massouridès, J. Polentes, P.-E. Mangeot, V. Mournetas , J. Nectoux,N. Deburgrave, P. Nusbaum, F. Leturc, L. Popplewell, G. Dickson, N. Wein, K. M. Flanigan, M. Peschanski, J. Chelly and C. Pinset - 2014
-
Peer J . DOI:10.7717/peerj.618
(previously submitted as preprint to PeerJ - June 2014)
V. Mournetas , Q. M. Nunes, P. A. Murray, C. M. Sanderson, D. G. Fernig - 2013
-
The heparin-binding protein interactome in pancreatic diseases
Pancreatology . DOI:10.1016/j.pan.2013.08.004
Q.M. Nunes, V. Mournetas , B. Lane, R. Sutton, D.G. Fernig, O. Vasieva
Reviews
1
- 2012
-
LIF-Dependent Signaling: New Pieces in the Lego.
Stem cell reviews . 8(1):1-15. DOI:10.1007/s12015-011-9261-7
M.-E. Mathieu, C. Saucourt, V. Mournetas , X. Gauthereau, N. Thézé, V. Praloran, P. Thiébaud, H. Bœuf
Preprints
7
- 2022
-
bioRxiv . doi.org/10.1101/2022.09.06.506756
C. Savary, ..., V. Mournetas , ..., L. Broutier - 2022
-
bioRxiv . doi.org/10.1101/2022.07.10.499454 (Now published in Plos One - November 2022)
S. Auger, V. Mournetas , H. Chiapello, V. Loux, P. Langella, J.-M. Chatel - 2022
-
A transition support system to build decarbonization scenarios in the academic community
HAL
N. Gratiot,... V. Mournetas ..., I. Michaud-Soret, - 2021
-
bioRxiv . DOI:10.1101/2021.08.24.457331 (Now published in eLife - September 2022)
M. R. F. Gosselin, V. Mournetas M. Borczyk, L. Bozycki, M. Korostynski, S. C. Robson, C. Pinset, D. C. Górecki, - 2019
-
bioRxiv . DOI:10.1101/720920
(Now published in Journal of Cachexia, Sarcopenia and Muscle - February 2021)
V. Mournetas , E. Massouridès, J.-B. Dupont, E. Kornobis, H. Polvèche, M. Jarrige, M. Gosselin, S. D. Garbis, D. C. Górecki, C. Pinset - 2017
-
bioRxiv . doi.org/10.1101/202010
(Now published in Nature Communications - January 2019)
P.E. Mangeot, V. Rissons, F. Fusil, A. Marnef, E. Laurent, J. Blin, V. Mournetas , E. Massouridès, T.J.M. Sohier, A. Corbin, F. Aube, C. Pinset, L. Schaeffer, G. Legube, F.-L. Cosset, E. Verhoey, T. Ohlmann, E. P. Ricci - 2014
-
Peer J . doi.org/10.7287/peerj.preprints.415v2
(Now published in PeerJ - October 2014)
V. Mournetas , Q. M. Nunes, P. A. Murray, C. M. Sanderson, D. G. Fernig
In preparation
2
Abstracts
1
- 2022
-
JID . doi: 10.1016/j.jid.2022.09.243
V. Mournetas , M. Fayyad-kazan, M. Dorr, R. Fitoussi, S. bourgoin, M. Seve, W. Rachidi
Other
2
- 2018
-
Cah. Myol. . doi: 0.1051/myolog/201817016
V. Mournetas , E. Massouridès, E. Kornobis, C. Pinset - 2014
- 2020
-
PostDoc
Skin, aging and exposome
Working with Prof. Michel Sève (TIMC-EPSP, UGA) & Dr Walid Rachidi (Biomics, CEA)
TIMC-EPSP, UGA, La Tronche, France
- 2020
-
9-month self-training
Graphical interface deployment to explore omics data
Database hosting on Ubuntu server, SQL database, Shiny interface, R programming - 2015 / 2019
-
A 4.5-year PostDoc
Duchenne muscular dystrophy: cells models & high-throughput approaches for developing therapeutic strategies
Team leader (muscular diseases): Dr Christian Pinset
Omics (transcriptomics, miRnomics, proteomics) analyses between healthy and disease during cell differentiation
Troubleshooting of high-throughput screening for drug discovery by RT-qPCR
Development of national and international collaborations:
- wet biology ( e.g. ENS Lyon, France and University of Portsmouth, UK)
- dry biology ( e.g. Dassault Systèmes, France and IBISC Paris Saclay, France)I-Stem, Genopole, Corbeil-Essonnes, France
- 2010 / 2014
-
A 4-year PhD project
Defining stemness of human embryonic stem cells: a systems biology approach
Supervised by Prof. David G. Fernig, Dr Patricia Murray and Prof. Christopher M. Sanderson
in silico: microarray meta-analysis, construction of protein-protein interaction networks
in vitro: development of a method to quantify lentiviral particlesUniversity of Liverpool, UK
- 2010
-
A 6-month work placement in research laboratory
LIF cytokine study in mouse embryonic stem cells
Managed by Jean-François MOREAU, supervised by Dr Hélène BOEUF
RT-qPCR, proteomics (iTRAQ)
UMR CNRS 5164 CIRID (National Centre of Scientific Research), Bordeaux, FRANCE
- 2009
-
A 3-month work placement in research laboratory
Cloning and characterisation of novel moss lipoxygenases
Managed by Prof. Yokota KAZUSHIGE, supervised by Prof. Mitsuo JISAKA
RACE-qPCR
Shimane University, Faculty of Life and Environmental Science, Matsue, JAPAN
-
A 6-month team project
Production and purification of polyhydroxybutyrate (PHB)
in collaboration with the Irstea (ex Cemagref)Clermont-Ferrand, FRANCE
2020
1 research article for the
Molecular Therapy - Methods & Clinical Development
2019
1 research article for the
Canadian Journal of Physiology and Pharmacology
- 2017 - 2019
-
Supervising undergraduate students during internship
3 Master2 students
- 2011 - 2013
-
Involvement in teaching undergraduate students during practical classes
Demonstration, marking and invigilating (total of 260 hours)
- 2005 - 2007
-
Teaching mathematics to secondary school students, private lessons
Lesson/test preparation and marking (total of 50 hours)
Session chairs:
2019
REVIVE annual meeting, Chantilly-Gouvieux, France
2014
Institute of Translational Medicine, Post-Graduate Symposium, Liverpool, UK
2013
Manchester Life Sciences PhD Conference, Manchester, UK
Other:
2019
Member of the “Fondagen Grants for PhD students” evaluation committee
Genopole, Evry, France
2017
Co-organiser of the
Genodoc
seminars for PhDs and PostDocs
Monthly seminars to highlight career paths
2015 - 2018
Volunteer for "1000 chercheurs dans les écoles"
Once a year seminars in high schools to explain what is research in biology (organised by the
AFM-Téléthon
)
Press articles (in French): L'Echo.info,
La Montagne
2014
Co-organiser of the 5th MSCA Annual Meeting
19th of September 2014, University of Liverpool
2014
Co-organiser of the 1st MSCA Young Investigators Workshop on lentivirus techniques
18th of September 2014, University of Liverpool
2014
Young Investigator committee member of the Mercia Stem Cell Alliance (MSCA) Network
2013 - 2014
Co-website designer/manager and co-social media manager of
Scouse Science Alliance blog
Website design and maintenance, social media advertisement (
LinkedIn
,
Twitter
)
2012 - 2014
Laboratory meeting organiser
Schedule preparation, dealing with requests and improvement process
2007 - 2009
Member of the school association of biological department
Website maker
Help to organise a Telethon (Television-Marathon) event in order to raise money for a charitable cause
2019 - Present
2016 - Present
2016 / 2019
2012 - 2016
2012 - 2016
2012 - 2016
Awards
4
1 best oral communication prize
(2016)
3 best poster prizes
(2012, 2014, 2014)
Grants
6
Travel Grant
(2019 - 1.500 euros - to go to the ISSCR2019)
Postdoc fellowship (Genopole)
(2018 - for 2 years - to hire someone)
'Crédit Agricole' grant, principal investigator
(2017 - 30,000 euros)
BioTherAlliance grant, principal investigator
(2017 - 10,000 euros)
Postdoc fellowship (Revive Consortium)
(2016 - for 2 years and 8 months)
'Fondation Maladies Rares'' GenOmics grant, co-applicant
(2016 - 65,000 euros)
- 2020
-
University degree in integrative biology (DUBII)
Unix, R, Python
Université de Paris, FRANCE
- 2014
-
PhD in Stem Cell Systems Biology
- 2010
-
Research Master qualifying for Doctoral studies specializing in Genetics and Physiology
Université Blaise Pascal, Clermont-Ferrand, FRANCE
-
Diplôme d’ingénieur (Postgraduate diploma) qualifying for Doctoral studies specializing in Biological Engineering
Microbiology, Biochemical Engineering, Biochemistry, Molecular and Cell Biology, Genetics, Enzymology, Micro-organism and Cell Culture, Metabolism, Protein Engineering
Ecole d’ingénieur Polytech’Clermont-Ferrand, Clermont-Ferrand, FRANCE
- 2007
-
A two-year university degree in Applied Mathematics
Analysis, Algebra, Numerical Analysis, Computer Sciences
Université François Rabelais, Tours, FRANCE
- 2020
- 2018
-
A 3-day course on R programming
INSERM, Paris, FRANCE
- 2012
-
A 1-day course on Quantitative Proteomics
followed by the ProteoMMX2.0 Conference, Chester, UK
- 2011
-
A 5-week Developmental Biology Course
Institut Curie and Université Pierre and Marie Curie) , Paris, FRANCE ,in collaboration with the Developmental and Regenerative Biology graduate program of HARVARD University
-
A 4-day course on mathematical modelling
co-organised by CPIB & SIGNET, University of Nottingham, Nottingham, UK
-
A 2-week course in Systems Biology: Morphogenesis & Spatial Dynamics
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Publication
I am very pleased to share with you our latest work on human myogenesis and DMD early onset modelling.
We have analysed time series omics on human pluripotent derived cells and found several interesting elements around mitochondria or fibrosis. But to us, the most important finding is that transcriptional defects appear at the somite stage, before the expression of skeletal muscle markers and before the expression of the muscular dystrophin.
If you are interested, you can find the entire manuscript here, as well as explore the entire datasets here.
Feel free to contact us for any questions or comments you have; we will be happy to discuss.
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Job offer
The fellowship amount is ranged from € 60,000/year to € 75,000/year (gross salary charged and taxes) according to the experience level of the candidate and is allocated for a maximum of 2 years and is exclusively intended to cover salary costs and related social security contributions.
Genopole-Evry is launching a new call for applications, the objective of which is to grant a fellowship intended to facilitate the return to France of a young researcher trained in France who has completed at least one post-doctoral internship abroad in order to accompany a research project within the Genopole biocluster.
This fellowship is strictly for the sole purpose of:
-A young French or foreign researchers trained in France. i. e. who have obtained their doctorate in France or who have completed at least 3 years of higher education in France.
-And who wish to return to France after a stay abroad.
Studying onset of Duchenne muscular dystrophy during myogenesis from human pluripotent stem cells to identify early markers and therapeutic targets
Duchenne muscular dystrophy (DMD) is the most common form of muscular dystrophy. It is a recessive X-linked monogenic myopathy caused by mutations in the dystrophin gene leading to the loss of a functional protein. Most DMD patients display severe phenotypes that usually appear in early childhood (2-5 years old). These patients, one boy on 3,300 boy births, have a progressive loss of muscle strength implying the use of wheelchair by age 12 and leading to premature death due to cardiac and respiratory failures in their late twenties.
The main role of the muscle long dystrophin isoform is to maintain the homeostasis of adult muscle fibres by participating to membrane stability during contraction. Many converging data and our recent discovery of the existence of an embryonic form of dystrophin specific to the early stage of differentiation in anthropoids lead to propose new functions of this protein during the onset of skeletal muscle differentiation.
Combining the use of cell models derived from human pluripotent stem cells which mimic the earlier phases of differentiation with the exhaustive expression analysis by next-generation-sequencing approaches, our team has been able to show that DMD cells present a specific and complex phenotype from 3 days of differentiation. More precisely, we have been able to identify around 300 genes significantly dysregulated in DMD skeletal muscle progenitors.
From the analysis of high-throughput sequencing data, the postdoctoral researcher will elaborate novel hypotheses on the DMD phenotype during the early phases of development that will be tested in vitro by functional approaches such as gain- and loss-of-function experiments. The goal is to find DMD early markers and/or novel therapeutic targets complementary to the dystrophin rescue. This project should lead us to rethink the dystrophin function(s) during human myogenic differentiation.
If you are interested to apply, please contact : Christian Pinset, MD, CNRS research director
at cpinset@istem.fr
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Symposium
- 1st MSCA young investigator workshop:
- Date: 18/09/2014
- Fees: £5 only
- Theme: lentivirus techniques for knock-in and knock-out approaches - Production & Quantification
- Technical and research presentations
- Opportunities for PhD students and postdocs to present their research via oral presentation
- Technical ressources will be accessible
- Abstract submission (posters & talks) deadline: 15/07/2014
- Registration deadline: 31/08/2014
- 5th MSCA annual meeting:
- Date: 19/09/2014
- Fees: £10 only
- Keynote speaker: Benjamin Dekel, University of Tel Aviv, Israel
- Opportunities for PhD students and postdocs to present their research (oral presentation, poster & 2 min poster teaser talk)
- Abstract submission (posters & talks) deadline: 23/08/2014 (EXTENDED)
- Registration deadline: 31/08/2014
The abstract submission form has to be sent to i.santeramo@liv.ac.uk.
Register via the online store.
For full program, click below:
Questions? Requests? Comments? Contact me!
Location
Last update: 12/02/2023